Biocompatibility Study of Lactobacillus Casei

Biocompatibility Study of Lactobacillus Casei BIOCOMPATIBILITY STUDY OF LACTOBACILLUS CASEI ISOLATED FROM CUCUMBER AND EVALUATION OF PROBIOTIC EFFECTS IN THE HUMAN GUT NANNU SHAFAKATULLAH* and M. CHANDRA Abstract: Probiotics are live microorganisms introduced orally in the gastrointestinal tract (GIT) that are able to contribute positively to the activity of intestinal microflora and therefore, to the health of its host. A variety of probiotic supplements are currently available in the market which target towards improving the balance and activity of the intestinal microflora. Probiotics must have robust survival properties in the gut in order to exert any beneficial health promoting properties. Many in vitro properties, such as adhesion, co-aggregation, aggregation, hydrophobicity, resistance to pH, bile, etc., are usually investigated to determine if a specific selected strain would be suitable as a probiotic. Lactobacillus casei has been isolated from raw cucumber and identified based on phenotypic and biochemical characteristics. The isolate was studied for its survival at acidic pH, bile salt, intestinal juice, gastric juice, co-aggregation, aggregation, different NaCl concentrations, the ir action against pathogens, and resistance to antibiotics. The organism has shown well resistance to antibiotics, gastric acids and bile digestion and also exhibited good adhesion to intestinal mucosa and aggregation properties. Key words: Biocompatibility, cucumber, gastrointestinal tract, Lactobacillus casei, probiotics Introduction: Probiotics are defined as â€Å"live microorganisms which when administered in adequate amounts confer a health benefit on the host†1. The use of probiotic bacteria for the health of human has been increased from the last decade due to the increased research on benefits of the probiotics for human. Knowledge of gut health and awareness of general health consciousness in human leads to the search of new probiotic bacteria2-7. It has been proved that irritable bowel syndrome, inflammatory bowel disease, and antibiotic-induced diarrhea that occur due to the imbalance in the intestinal microflora can be reversed by the intake of probiotics.Lactobacillus species are â€Å"Generally Recognized as Safe† (GRAS) microorganisms and they are the most commonly used microorganisms as probiotics and are the most desired intestinal microflora. It is important to study the biocompatibility of the lactobacillus species before using them as probiotics. Acidic pH, bile salts, and gastric and intestinal juice in the gastrointestinal tract (GIT) are the major stress factors that the probiotics should overcome in order to survive in GIT. Other than their survival capabilities, the probiotic microbes consumed should possess the capacity to adhere and colonize in the gastro intestinal tract. More the adherence capacity of the probiotics more is their chance to retain themselves in the GIT and provide positive effect to the consumer. In accordance with the FAO/WHO8 guidelines in order to prevent the transmission of antibiotic resistance genes from the probiotics to the intestinal pathogens it is recommended that the antibiotic resistance/susceptibility pattern of every probiotic strain (including bacteria with GRAS status) is to be determined. Due to the development of antibiotic resistant pathogens there is increased interest in the alternative antimicrobial strategies for treatment and prevention of infections by using probiotics and their antimicrobial metabolites. Hence, antimicrobial activity against pathogens is a desirable property of a potential probiotic strain. The present study was aimed at isolation, identification, characterization and biocompatibility study of the Lactobacillus strain isolated from cucumber. The biocompatibility properties were investigated throughin vitro assays. Material and Methods Isolation and Identification of Bacteria Fresh cucumber juice was prepared and 1ml of this was serially diluted to 10-5 to 10-6 and inoculated 0.1 ml on to lactobacillus MRS agar plates and incubated at 37 °C for 24-48 hours anaerobically. Gram’s staining, catalase activity, gas production from glucose, acid fast test, MRVP test, gelatine hydrolysis, oxidase test, growth in different NaCl was determined according to methods for lactic acid bacteria9-10. The identification work was done according to the methods described in Bergey’s Manual11 and the Prokaryotes. All the strains were maintained by weekly sub culturing from 48hrs MRS agar cultures. Growth characteristics at different temperature were monitored for 7 days period. Production of ammonia from arginine was done according to the method described by Abdel-Malek and Gibson12, Nitrate reduction was done as described by Gerhardt et al., 13. The isolates were maintained in MRS broth, stock cultures were stored on agar slants in refrigerator and also freez e dried and stored for longer period. Growth at acidic pH The growth behavior of culture isolate was observed at acidic pH to find the acid tolerance capacity of organisms. The Isolate was inoculated in MRS broth with pH 2 and 3 and incubation was done at 37 °C for 48-72 hrs. During these incubation time cells growth was observed and results were recorded. Transit Tolerance: 1. Simulated Gastric Juice The simulated gastric juice was prepared freshly by suspending pepsin 1:10000 (3g/L) (SRL) in sterile NaCl (0.5%) and the pH was adjusted to 2.0 and 3.0 respectively. This was filter sterilized using 0.45 µm filter. The L. casei was grown in de Man, Rogosa and Sharpe (MRS) broth at 37 °C for 24 h and centrifuged at 2,500 Ãâ€" g at 4 °C for 10 min. The collected cells were resuspended in sterile saline (0.5% NaCl) and inoculated into the simulated gastric juice (pH 2.0 and 3.0) at 108 cfu/ ml. The test was done in triplicates. Because the pH in the human stomach ranges from 1 (during fasting) to 4.5 (after a meal) and food ingestion can take up to 3 h, tolerance was assayed by determining the total viable count at 0, 1.5 and 3-h incubation in simulated gastric juice (pH 2.0 and 3.0). 2. Simulated Intestinal Juice The simulated intestinal juice was prepared freshly by suspending pancreatin (1g/L) in sterile NaCl (0.5%) and adjusted the pH to 8.0. This was again filter sterilized by using 0.45 µm filter. 1ml of the suspension of the L. casei was inoculated into 9ml of simulated intestinal juice (pH 8.0) and incubated at 37 °C. The test was done in triplicates. The survival rate was assessed by determining the total viable count at 0, 2, 4, 6 and 8hrs of incubation. 3. Bile Tolerance Bile plays an important role in the survival of bacteria in the small intestine. Food remains in the small intestine for around 4-6 hours14 till it gets absorbed. The L. casei was screened for its survival at different bile concentrations. The organism was inoculated into 10 ml MRS broth in test tubes and incubated at 37 ºC overnight in anaerobic condition. 100ÃŽ ¼l of active culture was inoculated into fresh MRS broth tubes with pH 6.5 containing 0.3%, 0.5% and 1.0% bile (CDH India). The bacterial survival was measured by MRS agar colony count by taking 100ÃŽ ¼l culture for 0, 30, 60, 90 and 180 min and aliquots spread onto MRS agar plates to calculate the CFU/ml. The experiment was determined in triplicate to calculate intra-assay variation. CFU/ml was recorded. Aggregation (Del Re et al., 2000) Aggregation assay was performed by growing the isolates in MRS broth for 24 hours anaerobically at 37oC. The cells were harvested by centrifugation at 5000 rpm for 15 min, at 4oC. The cells were washed twice and re-suspended in phosphate buffered saline (PBS) to give viable counts of approximately 108 CFU/ ml. Four ml of the cell suspension was mixed for 10 seconds in a sterile tube to determine auto aggregation during 5h of incubation, at room temperature. The upper suspension was used in each hour by transferring 0.1ml to another 3.9ml of phosphate buffer solution, and the optical density at 660nm was measured. Tests were carried out in triplicate and the results were averaged. The auto aggregation percentage was calculated by the formula: 1- (At/A0) X 100, where, At represents the absorbance at time t = 1, 2, 3, 4 or 5, and A0 the absorbance at t = 0. Aggregation abilities of microorganisms were screened by visual observation. Co-aggregation The bacterial cells were harvested by centrifugation at 5000 rpm for 15 min after incubation at 37 °C for 18h, washed twice and resuspended in phosphate buffered saline (PBS) to give viable counts of approximately 108 CFU /ml. Equal volumes (2 ml) of each cell suspension were mixed together in pairs by vortexing. Control tubes were set up at the same time, containing 4 ml of each bacterial suspension on its own. The absorbances at 660 nm of the suspensions were measured after mixing and after 5 h of incubation (6-9). The percentage of co-aggregation was calculated using the equation Handley et al. (1987) as, Co-aggregation (%) = [(Ax + Ay) /2) A (x+ y)] / [(Ax + Ay)/ 2] Ãâ€" 100 Where x and y represent each of the two strains in the control tubes, and (x + y) the mixture of isolate tested for co-aggregation. Antibiotic Sensitivity test Antibiotic sensitivity test of the isolate was performed by standard disc diffusion method (NCCLS 1999) towards thirteen antibiotics. The pure culture of L. casei suspension was spread on the MRS agar plates to form a uniform smear. Selected antibiotic discs were aseptically transferred on to the seeded plates. The diameters of the zone of inhibition were measured using antibiotic zone scale (Himedia India) after 24 h of incubation. The experiment was repeated thrice and the average inhibitory zone diameters were compared with the standards provided by the National Committee for Clinical Laboratory Standards. Diameters of inhibition zones were measured and results were expressed as sensitive, S (≠¥ 21 mm); intermediate, I (16-20 mm) and resistant, R (≠¤ 15 mm), respectively according to that described by Vlkovà ¡ et al., 15. Antimicrobial Activity Test Agar well diffusion method16 was used to determine the inhibitory capacity of the L. casei against pathogenic strains such as Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Bacillus subtilis. The isolate and pathogenic strains were incubated in MRS agar medium at 37 °C for 24 to 48 h. Result and Discussion Physiological and Biochemical Characterization The isolate was subjected to Gram’s staining and it was examined under light microscope. The strain gave blue- purple color with staining; hence it was Gram positive bacteria. Isolate was tested for catalase activity. It was catalase negative (do not show catalase activity). To test the gas production from glucose test tubes were observed for 5 days. The Isolate shown no gas production this indicates its homofermentative nature (Table 1). Another criterion for the identification the isolate was the ability of growth at different temperatures (Table 2). From the results of 5 days observation the isolate showed growth at 15-50  °C. Growth at different NaCl concentrations was observed. The isolate has the ability to grow at 2-6% NaCl concentration. Arginine hydrolysis test was another step to follow the identification procedure. The isolate which gave the bright orange were accepted that they can produce ammonia from arginine. The yellow colour indicated negative arginine hydro lysis. The isolate has shown -ve for arginine hydrolysis. Hydrolysis of starch was negative by isolate. The isolate was non motile, non spore forming. The most useful test for the determination of strain differences is carbohydrate fermentation. Twenty one (other than glucose) different carbohydrates were used for identification. They gave different fermentation patterns when they were compared. The patterns are showed in Table 3. Resistance to acidic pH Being resistant to low pH is one of the major selection criteria for probiotic strains17-18. Since, to reach the small intestine they have to pass through from the stressful conditions of stomach19. Although in the stomach, pH can be as low as 1.0, in most in vitro assays pH 3.0 has been preferred. Due to the fact that a significant decrease in the viability of strains is often observed at pH 2.0 and below20. Sudden decrease in the survival rate of the isolate has been observed at pH 2. At the third hour the survival rate reduced below 5%. At pH3 more than 10% survival has been observed after 3 hours of incubation. Tolerance against Bile The isolate was screened for its ability to tolerate the bile salt. Although the bile concentration of the human gastrointestinal tract varies, the mean intestinal bile concentration is believed to be 0.3% w/v and the staying time is suggested to be 4 h. Strain was screened for 3 hours in 0.3%, 0.5% and 1.0% of bile salt for its survival. The cfu values were observed. According to the results the isolate was resistant to 0.3% and 0.5% bile salt. Whereas sudden fall in the number of survival organisms has been observed at 1.00% bile. The survival rate reached to 5% at the end of 3 hours of incubation at 1.00% of bile. Tolerance to Gastric Juice The degree of gastric juice resistance exhibited by isolate was determined and results (Figure 4) showed that >75% of survival has been observed in gastric juice at pH 3 for 1.5 hours of incubation, whereas at pH2 the survival rate was >30% for 1.5 hours of incubation. But at 3 hours of incubation the survival rate at pH 3 reached to Tolerance to Intestinal Juice The isolate was tested for its ability to grow in intestinal juice. It appears that the strain exhibited good resistance to intestinal juice at pH 8 for four hours of growth (Figure 5). Good multiplication of all the isolates has been found at 6th hour of incubation. Aggregation On the basis of sedimentation characteristics aggregation capability of the isolate was tested. L. casei has exhibited good amount of aggregation during the test time of 5 hours (Figure 5). Co-aggregation The co-aggregations of L. casei with five pathogenic bacteria were examined. Results were expressed as the percentage reduction after 5 h in the absorbance of a mixed suspension compared with the individual suspension. Good co-aggregation of L. casei with S. aureus has been seen. There was no co-aggregation between L. casei and B. subtilis. 2-6% of co-aggregation has been seen with E. coli, K. pneumonia and P. aeruginosa (Figure 6). Antibiotic Sensitivity test The determination of antibiotic sensitivity of the isolate is an important prerequisite prior to considering it safe for human and animal consumption. The isolate was subjected to antibiotic susceptibility test. The results are given in Table 4. The isolate was resistant to most of the antibiotics used. According to earlier reports, specific antibiotic resistance traits among probiotic strains may be desirable21. It has been said by many authors that probiotics should be resistant to certain antibiotics when used along with antibiotics to prevent gastrointestinal disorders. Whereas others claim that antibiotics resistant probiotics used may serve as host of antibiotic resistance genes, which can be transferred to pathogenic bacteria. Antimicrobial Activity Test Antimicrobial activity helps to select the potential probiotics strains. Antimicrobial activity usually targets the intestinal pathogens. The isolate was examined for antibacterial activity. L. casei was grown with indicator microorganisms, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Bacillus subtilis. The antibacterial effect on the indicator microorganisms was determined by diameter of inhibition zones. Lactobacilli casei has a high ability to inhibit the growth of pathogenic microorganisms. The degree of inhibition was highest in S. aureus, whereas the inhibition was moderate in E. coli, K. pneumonia and P. aeruginosa. The isolate could not inhibit the growth of spore forming B. subtilis. Conclusion: Lactobacillus casei has shown good survival in acidic pH, different bile concentrations, gastric juice and intestinal juice. The organism exhibited good survival in the presence of different antibiotics. The isolate is also able to inhibit the growth of different pathogenic microorganisms examined. All these characteristics of the organism will help it to survive in the stomach and proliferate in the intestine. This will help strains to reach the small intestine and colon and contributing to the balance of intestinal microflora.

War on Poverty: Role of the Privileged People †S.C. Aggarwal

War on Poverty: Role of the Privileged People S C Aggarwal Delhi: Shipra Publications, 2007, pp. 135, Rs. 350. 00, ISBN 978-81-7541-378-8 S. C. Aggarwal’s book – War on Poverty: Role of the Privileged People, takes a very informal and straightforward approach in explaining the prevalence and significance of poverty in India. Even though the issue is widely discussed amongst leaders and the normal public alike, there is little change in the conditions of the poor over the past few decades.The author takes a very structured approach in explaining the situation of poverty in India, starting from the very basics, by providing important facts and some frank admissions by well-known government authorities, economists and personalities. Being an IRS officer himself, he goes on to admit that there have been flaws in Government policies in the past and suggests that the misguidance can be corrected if help is received in the future.He presents the reasons for the prevalence of p overty in India and highlights the negligence of ancestral villages by people and the lack of new programmes by economists as the main contributing factors for the same. The author has done a great job in giving various methods to remove poverty in a very simplistic manner, enabling its understanding even by the layman. Pointers to multiple approaches to people from varied professions, asking for their support in the rise of the poor and the eventual growth of the economy are some of the prime take-aways from this book.Appeals to people from all strata in the society have been made, personally requesting each of them for help in improving the conditions of one or two villages, especially their ancestral village. Prominent film makers, editors, interviewers, television programmers, TV channel owners, accountants, teachers, etc. are some of the people that the author has reached out to, suggesting ways in which each of them can contribute towards the removal of poverty. S. C.Aggarwalà ¢â‚¬â„¢s central idea is based on the effort that these intellectuals must strive to deliver in order to remove poverty from their respective native villages. He suggests that only a truly determined effort from their side, irrespective of their occupations, will be sufficient to remove poverty and improve the conditions of more than one third of the impoverished segment of our population. Various schemes and plans have been extensively discussed for each of them, enlightening them on how they may contribute towards this cause.Several queries have been raised to economists and intellectuals, making them realize their responsibilities towards the poor, through a series of questions which are directly related to their line of work. These questions force them to think, and hence take action towards poverty removal. Improving the condition of the schools which made them what they are, providing basic low cost health facilities in order to provide the spread of diseases, educating the pu blic about healthy living are some of the issues addressed to all individuals.For a start, each individual may solve one or two problems of his village, or help improve the conditions of buildings and roads in the village. The discussion on how we may make our village problem-free, with a focus to starting personalised schemes for the welfare of the people, and the resulting benefits of the same provokes a thought in the minds of the reader. In addition to the overly simplistic methods suggested which will help achieve a GDP greater than 7%, the author has also kept in mind the situation encountered in taxing unaccounted income in the book.This issue of black money, which is one of the key reasons for increase in poverty, has been intelligently dealt with, by providing practical solutions that will instigate people to declare the same during taxation. This will not only allow for increased taxes leading to increased income to be directly used in poverty eradication, but will also le ad to lower number of loans and reduction of dishonesty by people when declaring taxes.Also, providing benefits to tax payers or those with no interest payable, to those using their own funds to buy cars/property, alongside introducing added taxes for poverty removal on each individual’s income or purchase/sale of shares, are some of the measures suggested that may help better manage the problem of poverty. These methods will lead to a positive outlook in the minds of the people, encouraging them to contribute more than what they are already contributing towards poverty removal. Another useful tip is the setting up of Poverty eradication banks and poverty eradication funds by the Government and prominent editors respectively.In short, equal participation of people from all walks of life in the fight against poverty is the only way our country will be able to establish itself a global leader. S. C. Aggarwal concludes the book with a humble prayer to all, comparing poverty to v arious metaphors, in order to realize the various ways in which each one of us interprets poverty. People are requested to give a helping hand to their fellow country-men, and to lift them from their dire states in society, to that that will help them procure the basic amenities of life for themselves and their families.One must take up the responsibility of their own villages, by trying to connect to the emotions that each individual has towards the place of his birth, where he/she grew up or where his/her parents resided/ are still residing. The author suggests that their responsibility does not end at removing poverty only from their respective villages. Rather, one must spread this idea to other people, at least three more, so that they may eradicate poverty from their own villages, along with spreading this idea as well.However, the basic problem that may be encountered in order to administer these ideas is that it is dependent of too many people, and hence it may be difficult to track the progress of every individual towards his village. Also, the methods suggested in the book seem a little farfetched, and hence will require patience for their achievement. All in all, the book is a good read for people wanting to do something for their societies by enriching us with the basics that each of us must contribute for its development.

American education Essay

I grew up in America and received an American education. I have an American wife and children and I love the life I have built for myself in America. My dilemma is that I am, by birth, beholden to become the chief of an African tribe upon the death of my father who was chief of the tribe. My loyalties are torn between my life in America and my responsibility to a tribe I have never known in a land I don’t understand, and in a role of leadership which I have never experienced. I must also consider my wife and children; I must make a decision either to shirk my responsibility to the tribe or to take my family to a very strange, possibly dangerous land and upset our lives completely. Because it seems to me that the cultural and technological changes that are impacting Africa in the twenty-first century are complicated and profound, I feel a strong sense that I should serve the tribe. It would be very difficult to give up my life in America because the life described n Africa in the film sounds demanding and very alien to the social customs of America. However, it seems that refusing to provide wisdom and knowledge and leadership to the tribe due to fear of change or personal selfishness would be morally wrong. Because I have had a good education in America, I will be able to help the African tribe in many ways. There’s no reason why I shouldn’t be able to conduct travels to America and do business on behalf of the tribe in America or work to provide opportunities for those in the African tribe who seek it to become educated in America. In some ways, the responsibility to the tribe is not only to facilitate a transition into the twenty-first century but to uphold the promise of my father who, as chief, promised the tribe that I would serve upon his death. Taking my family to Ghana will be a very difficult and very troublesome action which is a sort of sacrifice that will be necessary in order for me to do what is morally and practically the right thing to do. It would be wrong to turn my back on the tribe, but it is potentially dangerous and destructive to uproot my family and take them to a foreign land. On the other hand, the opportunity may well prove to be a good one for both myself nad my family. The land may show us mysteries and cultural differences which will make us better people, and maybe even happier people. There may be issues other than those of service to the tribe. After-all, I will be chief and me and my family will be held in high esteem by the tribe; we will be important and influential and we may find that our new roles are right for us after-all. Going to Africa will also allow me and my family a chance to find out about my ancestry and the history of the tribe. It could be that certain tribal traditions and tribal wisdoms will prove important for Westerners to understand and that my role as a â€Å"bridge† will work both ways: I may impart wisdom about modern ideas and technologies to the tribe but I may be able to use traditional tribal ideas and cultural wisdoms to elucidate problems which face the â€Å"modern† world. The final factor which weighs in my decision is the fact that the tribe has already pronounced me chief and accepted me as chief. In this sense, the tribe is also my â€Å"family’ they are simply family members which I have not yet become attached to and involved with, but it is important for me to make a self-sacrifice on behalf of the tribe. I would choose to become Chief of the tribe in order to provide a cultural ‘bridge† from the African traditions to the modern changes which are going to impact the tribe, whether they want them or not and whether or not they are prepared. I feel a sense of responsibility to my father’s reputation and to the tribe, as well as to myself and my family.

Should The Constitution Be Ratified For The Future

Throughout American history the constitution has been the framework for democracy. Written in 1787, the constitution was a great conception for the thirteen colonies. Now two-hundred and twenty-eight years later the United States is not a county of freedom fighting European-Americans. In this diverse and modern society concerns have come to surface as to whether the constitution should be ratified for the future to come. Ratifying the constitution sounds like a good notion, but is nearly impossible to do. I believe the constitution should stand as is but allow another document arise that corrects the loop holes of the constitution. When the colonist were drafting the constitution they couldn’t have imagined the tremendous growth we have achieved today. With innovation comes conflict. Many citizens feel the United States gives an illusion of freedom. Today the biggest conflicts are centered on basic rights spelled out in the constitution. It’s no secret the National Association of Surveillance illegally obtains information from the electronic devices of United States citizens. The actions of the NSA violate the 1st, 4th, 5th, and 9th amendment rights. The NSA’s use of information impedes on the first amendment in terms of freedom of press. For a journalist the source is the key, and the key stays confidential. With the NSA collecting digital trails there is a higher risk for whistle blowers to be charged with criminal act or even assassinated. The courts stand by the NSA, forShow MoreRelatedThe Constitution Of The United States Essay1654 Words   |  7 PagesArticles of Confederation. In do ing so, they drafted a Constitution that would serve as the law of land for that thirteen states and any others that would join the Union. The Framers designed the Constitution for the purpose that it could be amended in the future. This is because they would not be able to foresee all the circumstances that the state would face in the future, therefore they wanted it to be able to be easily amended in the future. Today, lawmakers in Washington D.C. are trying to utilizeRead MoreTheu.s. Constitution And The Constitution1612 Words   |  7 PagesThe U.S Constitution is one of the most, if not the most, important documents ever written in political history. The constitution established America’s national government and provided the fundamental framework for the present and future legal parameters by which the american people would be governed by. The Constitution was officially signed September 17, 1787 in Philadelphia during a constitutional convention. In order for the const itution to actually be an official document, it had to have beenRead MoreThe Rartifiation of the Us Constitution1046 Words   |  5 PagesQuestion: What were the major arguments used by each side (the supporters and the opponents) in the debates over the ratification of the U.S Constitution? In the year 1787, early America, officials and delegates came together to form a constitution that would restore the Articles of Confederation. The Articles of Confederation was the attempt at creating a government for the newly independent America. But, it soon became clear that the document was not strong enough toRead MoreThe Rights Of The Citizens Of A Country973 Words   |  4 Pagesshall never do. These limitations serve to protect freedoms of religion and speech, a free press, free assembly, and free association, and the right to keep and bear arms. The U.S Bill of Rights is a document that officially became part of the constitution in 1791, consisting of the Constitution’s first ten amendments. It is the embodiment of the one true revolutionary idea in all history, and played a major role in laying the framework for rights as citizens of America. It was written by James MadisonRead MoreU.s. Constitution And The Constitution1437 Words   |  6 PagesThe U.S. Constitution, as adopted by the Philadelphia Convention on September 17, 1787, sets out three distinct branches of national government and provides powers to each that serve as a check on the others. The branches include the executive branch: the president, the legislative branch: congress and the judicial branch: the Supreme Court. The Constitution is referred to as a living document because it is open to constant change. Additionally, the Constitution is open to constant interpretationRead MoreThe United States Congress On The Rights Of The Child ( Crc )1506 Words   |  7 PagesThe United States’ Senate should ratify the United Nation’s human rights treaty, The Convention on the Rights of the Child (CRC). The CRC is the most widely ratified human rights treaty with over 193 states parties ratifying it (unicef.org). The CRC was adopted by the UN General Assembly on November 20th, 1989 and promotes the rights of children worldwide (childrightscampaign.org). The treaty recognizes that children have certain unalienable rights. These rights are to develop physically, mentallyRead MoreThe Constitution : A Powerful Movement Throughout The American History Essay1107 Words   |  5 Pagesalways been a powerful movement throughout the American history. Even as the Constitution was ratified on the 21st of June in 1788, federalists, and antifederalists fought against the opponent’s ideals. One side wanted the new country to have a strong centralized government (federalists), and the other side (antifederalists) believed in a smal ler central government, and state sovereignty. Many states didn’t ratify the Constitution unless a â€Å"Bill of Rights† was added guaranteeing unalienable rights theRead MoreAlexander Hamilton The Federalist Paper Summary1316 Words   |  6 PagesThe Constitution of the United States has not always been the establishment of our government. The Articles of Confederation, America’s first government, was a loosely binding pact formed between the first thirteen states of America, the existence of which did not even span a decade. Numerous issues including limited Congressional powers and no singular leader for the country contributed to the formation of our current constitution. However, in order for the constitution to be adapted as the newRead MoreAp Gov Unit 31006 Words   |  5 PagesWhat important purposes are served by the right to amend the Constitution guaranteed by Article V? Evaluate James Madison’s claim in Federalist 43 that the Constitution â€Å"guards equally against the extreme facility which would render the Constitution too mutable, and that extreme difficulty which might perpetuate its discovered faults.† Some critics of the amending process contend that amendments, once proposed, should be submitted to popular vote, bypassing state legislatures. Do you agree or disagreeRead MoreThe American Revolutionary War ( 1775-1783 ) Essay1671 Words   |  7 Pagesthe United States. The purpose of the Articles was to provide a general government of the 13 colonies that had won their freedom from British rule and to bring unity of the new states. The Articles of Confederation basically implied that every state should have its own independence, and operate within their own government any way they wanted. That wasn’t very smart considering that these colonies weren’t connected in any way. The Articles of Confederation undoubtedly had good virtues but was concerned